Journal article

Infectivity of plasmodium falciparum in malaria-naive individuals is related to knob expression and cytoadherence of the parasite

DI Stanisic, J Gerrard, J Fink, PM Griffin, XQ Liu, L Sundac, S Sekuloski, IB Rodriguez, J Pingnet, Y Yang, Y Zhou, KR Trenholme, CYT Wang, H Hackett, JAA Chan, C Langer, E Hanssen, SL Hoffman, JG Beeson, JS McCarthy Show all

Infection and Immunity | AMER SOC MICROBIOLOGY | Published : 2016

Abstract

Plasmodium falciparum is the most virulent human malaria parasite because of its ability to cytoadhere in the microvasculature. Nonhuman primate studies demonstrated relationships among knob expression, cytoadherence, and infectivity. This has not been examined in humans. Cultured clinical-grade P. falciparum parasites (NF54, 7G8, and 3D7B) and ex vivo-derived cell banks were characterized. Knob and knob-associated histidine-rich protein expression, CD36 adhesion, and antibody recognition of parasitized erythrocytes (PEs) were evaluated. Parasites from the cell banks were administered to malaria-naive human volunteers to explore infectivity. For the NF54 and 3D7B cell banks, blood was collec..

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Grants

Awarded by Atlantic Philanthropies


Funding Acknowledgements

This work, including the efforts of Michael F. Good, was funded by The Merchant Foundation. This work, including the efforts of Michael F. Good, was funded by Department of Health vertical bar National Health and Medical Research Council (NHMRC) (597476). This work, including the efforts of Michael F. Good and James S. McCarthy, was funded by Department of Health vertical bar National Health and Medical Research Council (NHMRC) (1037304). This work, including the efforts of James G. Beeson, was funded by Department of Health vertical bar National Health and Medical Research Council (NHMRC) (637406). This work, including the efforts of James G. Beeson, was funded by Department of Health vertical bar National Health and Medical Research Council (NHMRC) (1077636). This work, including the efforts of James S. McCarthy, was funded by Department of Health vertical bar National Health and Medical Research Council (NHMRC) (496636). This work, including the efforts of Michael F. Good, was funded by Atlantic Philanthropies.